Testing the Validity of “Old Wives Tales” About Fixation of Tissue Cultured Cells
نویسنده
چکیده
Over the years, many ideas have been handed down from one technologist to another about what to do and what not to do when fixing cells and tissues. This set of experiments takes into account certain conditions involving the age of the prepared primary fixative, length of time in primary fixative, length of time en bloc stain is on tissue cultured cells and the effect of washing the cells before fixation with phosphate buffered saline (PBS).
منابع مشابه
Isolation and Cultivation of Adult Human Keratinocyte Stem Cells for Regeneration of Epidermal Sheets
Background: Keratinocyte stem cell is one of the adult stem cells that inhabits the skin and contributes to skin function and renewal. Adult stem cells are best defined by their capacity to self-renew, and to maintain tissue function for a long period of time. These findings indicate the importance of these cells for clinical applications including regenerative medicine, tissue engineering and ...
متن کاملCharacteristics of Human Endometrial Stem Cells in Tissue and Isolated Cultured Cells: An Immunohistochemical Aspect
Background: The aim of this study was to investigate the percentage of the stem cells population in human endometrial tissue sections and cultured cells at fourth passage. Methods: Human endometrial specimens were divided into two parts, one part for morphological studies and the other part for in vitro culture. Full thickness of human normal endometrial sections and cultured endometrial cells ...
متن کاملHistological study of cartilaginous cells in the body of penis in one-humped camel (camelus dromedarius)
BACKGROUND:Several investigations showed cartilaginouscells in fibrous tissue of the free part of the penis in one humpedcamel. OBJECTIVES: The aim of this study was accurate assessmentof existence of cartilaginous cells in penis shaft of onehumpedcamel. METHODS: Six camel penises from maturedcamels more than 3 years-old were collected from an abattoir.Different specimens were prepared from eac...
متن کاملBiomechanical Comparison Between Bashti Bone Plug Technique and Biodegradable Screw for Fixation of Grafts in Ligament surgery
Background: Ligament reconstruction is a common procedure in orthopedic surgery. Although several popular techniques are currently in use, new methods are proposed for secure fixation of the tendon graft into the bone tunnel. Purposes: We sought to introduce our new technique of Bashti bone plug for fixation of soft tissue graft in anterior cruciate ligament (ACL) reconstruction and to compar...
متن کاملThe survey of knowledge and practice of faculty members about validity and reliability of exams
Introduction. Measuring is the foundation of evaluation. The main way for evaluation of education progression is testing the exams and every test must have identity, validity, reliability and be easy to perform. More precision to preparing the exams can improve its validity. There are different ways for measuring validity and reliability in testing. Do the faculty members know about these metho...
متن کامل